Real-time quantitative polymerase chain reaction (QPCR) for the identification and quantification of Streptococcus Mutans in saliva and dental biofilm in children
Real-time quantitative polymerase chain reaction (QPCR) for the identification and quantification of Streptococcus Mutans in saliva and dental biofilm in children
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Introduction: the objective of this study was to use real-time qPCR to identify and quantify the Streptococcus mutans species in samples of saliva and dental The study of geomagnetic jerk from 2010 to 2021 based on hourly mean data from global geomagnetic observatories biofilm.Methods: 27 children were randomly chosen with the following criteria: 8 years of age, low socio-economic levels, residing in the northern metropolitan area of Santiago de Chile; they were asked to attend an appointment while fasting with no teeth brushing for at least 12 hours, in order to collect non-stimulated saliva and a pool of supragingival dental biofilm of all the mesio-vestibular sides of anterior and posterior teeth.The amount of S.mutans in the samples was quantified by qPCR using primers that amplify a fragment of the gtfB gene of Metabolic effects of two high-intensity circuit training protocols: Does sequence matter? S.mutans.
Results: the amplification showed 98% efficiency with a fluorescence of 3.36 cycles.The melting curve presented a single maximum at the same temperature for all samples.Conclusion: the methodology allows the specific identification and quantification of gene gtfB of S.mutans in saliva and dental biofilm in a quick and reliable manner, contributing to the identification of individual cariogenic risk.